HEAD OF THE DEPARTMENT


Prof. PÉTER NAGY

Head of Department

CV


Department of Molecular Immunology and Toxicology (MITO)

Patient care

Our group mainly focuses on research projects, although we routinely carry out measurements for diagnostic purposes.
• We determine the concentration of methotrexat (MTX) from blood plasma samples of cancer patients in order to predict potential side effects and for monitoring the pharmacokinetics of the drug.
• New experimental methods for the determination of various tumor and other biological markers are currently under development in our laboratory. Following careful validation, we are planning to make these techniques available for diagnostics and clinical care.


Research Advisory Board

• Internal Research Committee
o Scientific Director
o Director of Innovation and Development
o Deputy Director General
o Clinical Director
o Director of Center of Surgery
o Director of Medical Oncology
o Director of Diagnostics and Imaging
o Director of the Center of Pathology

• External Research Advisory Board
o Professor Peter Boyle, Director of iPRI
o Professor Gunnar Saeter, Director of Research, Oslo University Hospital
o Professor Ulrik Ringborg, Director Cancer Center, Karolinska Institutet

Research Departments at the National Institute of Oncology

• Pathogenetics
• Molecular Genetics
• Molecular Immunology and Toxicology
• Experimental Pharmacology
• Biochemistry
• Radiation Biology
• Clinical Research

Current Research Programmes at the National Institute of Oncology

• Analysis of genetic diversity in the Hungarian population
• Discovering new targets to develop novel therapies
• Elucidation of molecular pathways responsible for treatment resistance
• Identification of new molecular markers for diagnosis and response to therapy
• Molecular diagnostics facility to ensure precision medicine to become a success. (molecular diagnostics, imaging, novel genetic based diagnostic panels, functional proteomics, innovative biomarkers, analyses of circulating tumor cells or exosomes etc)
• Prevention and early detection of hereditary cancers (breast, colorectal)

Clinical Research

• Wide scale of high profile research activities
o Both prospective and retrospective studies
o Increasing number of clinical trials and patients enrolled in these trials
 In 2015: 93 with 894 newly enrolled patients
 In 2016: 111 with 1062 newly enrolled patients
o Sponsored trials
o Collaborations with leading agencies (EORTC, International Breast Cancer Study Group etc)
o Investigator initiated and academic trials

Basic Research

• New basic research groups
o Department of Molecular Immunology and Toxicology
o Experimental Pharmacology
• New directions in basic cancer research
o Functional Proteomics
o Redox tumor-biology
o Development of novel therapies
o Mechanistic insights into regulation of cellular signaling from new angles
o Drug-peptide conjugates

Translational Research

National Oncogenomic project
• Coordinating role for NIO
• In the framework of this project—pioneering in the Eastern European context—the following objectives are aimed to be achieved by the consortium:
• Genomic examinations of solid organ and hematopoietic tumors in Hungarian patients.
• Identification of "driver" genes responsible for tumor phenotypes and the associated signaling pathways.
• Classification of the tumors’ new molecular genetic alterations using Big Data analyses.
• Detection of specific genetic alterations of individual patients.

National Excellence Program
• Innovations in breast cancer care
The ultimate goal of the project is to develop and implement a precision medicine model for the treatment of breast cancer by harmonizing surgery, radiotherapy, medical oncology, imaging, molecular diagnostics and basic research endeavors.

Wide scale of platforms to bridge clinical and basic research activities
o Seminar series
o Conferences
o Journal clubs
• Increasing number of collaborative projects and publications
o For example: Development of novel functional proteomics techniques to study resistance mechanisms for targeted therapies

Epidemiological Research

• Risk estimation of smoking and drinking patients
• Hereditary risk of cancer in breast, ovarian, colon and prostate cancer patients
• Identification of gene polymorphisms in breast, colorectal, head and neck and prostate cancers

Research Infrastructure at the National Institute of Oncology

• Designated Research Buildings
• Core Facilities
o Next Generation Sequencing:
 Roche FLX Genome Sequencer System, Illumina MiSeq System,
o Mass spectrometry: Thermo Orbitrap Fusion and LTQ XL mass spects
o Confocal microscope: NIKON Eclipse 80i C1 confocal microscope
o Flow cytometer: Beckman Coulter Cell Lab Quanta SC.
o DNA chip and high throughput protein expression profile analyzer
o Research Support Services
o Biobank (new centralized system development nearly finished)
o Animal House at Special Pathogen Free level:
 3000 live animal
• 5000 used annually
• 1/3 of the animals are immune suppressed
• Over 50 different tumor cell lines with 6-8 different inoculation sites
o Mechanical and electronics facility
o Isotope laboratory
o Research supplies warehouse
o Designated unit at the finances department

Research Output at the National Institute of Oncology

The number of annually published peer-reviewed articles, books, book chapters is >125 with a cumulative annual impact factor of > 600 in 2017 and 2018.

Research interest

The Department of Molecular Immunology and Toxicology was founded in 2011 and introduced a new point of view within the research platform of the National Institute of Oncology. The main motivation of our studies is to investigate the role of free radicals and reactive oxidizing/reducing species in tumor biology. Reactive Oxygen Species (ROS) and Reactive Sulfur Species (RSS) are standing in the focus point of our efforts, with special regards to their function in oxidative stress (e.g. chemo- or radiation therapy) and redox signaling (identification of therapeutic targets and unraveling of drug resistance pathways).
Targeted therapeutical approaches revolutionized the care of cancer patients, in a sense that unlike traditional treatments, they are designed to specifically eliminate tumor cells, exploiting their molecular fingerprints. According to a widely accepted perception, post-translational modifications, originating from protein-protein interactions, alter the functions of proteins thus affecting onco-signaling of cancer cells. Beside the thoroughly examined phosphorylation cascades, oxidation induced modifications are emerging participants of the regulation of signaling pathways.
Malignant cells overexpress ROS and RSS species compared to their healthy counterparts. Increased ROS and RSS flux suggest that anomalies in redox regulated signaling processes is a hallmark feature of tumor biology, providing potential therapeutic targets. This innovative approach may gain special importance for cancer types with no existing targeted therapy. In the framework of our research, we make attempts to facilitate the treatments of these severe conditions, by better understanding the molecular background of the underlying biology.

Our people

Our research group consists of experienced chemists, biologists and biochemists, collaborating on multidisciplinary projects on a daily basis.

• Prof. Peter Nagy, DSc.
Scientific Director
Head of department
Biochemist
Contacts: peter.nagy@oncol.hu
(1)-224-8600 / Extension 1170 or 3644

CV

• Zsuzsanna Lénárt
administrator
lenart.zsuzsa@oncol.hu
(1)-224-8600 / 2170
• Dr. Katalin Erdélyi
biologist
• Dr. Beatrix Kotlán
biologist
• Dr. Éva Dóka
chemist
• Tamás Ditrói
chemist
• Ágnes Czikora biologist
• Dorottya Garai
biologist, PhD student
• Klaudia Galambos
MSc student of biology, ELTE university
• Noémi Balog
leading laboratory assistant
(1)-224-8600 / 1180
• Attila Nagy
chemistry laboratory technician

Recent publications

* corresponding author

 

Tamás Ditrói, Attila Nagy, Diego Martinelli, András Rosta, Viktor Kožich, Péter Nagy*

Comprehensive analysis of how experimental parameters affect H2S measurements by the monobromobimane method

Free Radical Biology and Medicine (2019) 136, 146-158.
PubMed Link

 

John L. Wallace, Péter Nagy, Troy D. Feener, Thibault Allain, Tamás Ditrói, David J. Vaughan, Marcelo N. Muscara, Gilberto de Nucci & Andre G. Buret

A Proof-of-Concept, Phase 2 Clinical Trial of the Gastrointestinal Safety of a Hydrogen Sulfide-Releasing Anti-Inflammatory Drug

British Journal of Pharmacology (2019) Epub ahead of print
PubMed Link

 

Katalin Éva Sikura, Tamás Szerafin, László Potor, Melinda Oros, Péter Nagy, Gábor Méhes, Zoltán Hendrik, Abolfazl Zarjou, Anupam Agarwal, Niké Posta, Roberta Torregrossa, Matthew Whiteman, György Balla, József Balla

Hydrogen sulfide abrogate heart valve calcification: implications for calcific aortic valve disease

British Journal of Pharmacology (2019) Epub ahead of print
PubMed Link

Joseph Lin, Masahiro Akiyama, Iris Bica, Faith T. Long, Catherine F. Henderson, Robert N. Goddu, Valeria Suarez, Blaine Baker, Tomoaki Ida, Yasuhiro Shinkai, Péter Nagy, Takaaki Akaike, Jon M. Fukuto, and Yoshito Kumagai

The Uptake and Release of Polysulfur Cysteine Species by Cells:  Physiological and Toxicological Implications

Chemical Research in Toxicology (2019) 32, 447-455.
PubMed Link

Hisyam Abdul Hamid, Akira Tanaka, Tomoaki Ida, Akira Nishimura, Tetsuro Matsunaga, Shigemoto Fujii, Masanobu Morita, Tomohiro Sawa, Jon M. Fukuto, Péter Nagy, Ryouhei Tsutsumi, Hozumi Motohashi, Hideshi Ihara, and Takaaki Akaike

Polysulfide stabilization by tyrosine and hydroxyphenyl-containing derivatives that is important for a reactive sulfur metabolomics analysis

Redox Biology (2019) 21, 101096.
PubMed Link

Péter Nagy*, Günter Schwarz and Stanislav Kopriva

Highlighted mechanistic aspects in the chemical biology of reactive sulfur species

British Journal of Pharmacology (2019) 176, 511-513.
PubMed Link

Virág Bogdándi, Tomoaki Ida, Thomas R Sutton, Christopher Bianco, Tamás Ditrói, Grielof Koster, Hillary A Henthorn, Magda Minnion, John P Toscano, Albert van der Vliet, Michael D Pluth, Martin Feelisch, Jon M Fukuto, Takaaki Akaike and Péter Nagy*

Speciation of Reactive Sulfur Species and their Reactions with Alkylating Agents: Do we have any clue about what is present inside the cell?

British Journal of Pharmacology (2019) 176, 646-670.
PubMed Link

Christopher L. Bianco, Takaaki Akaike, Tomoaki Ida, Péter Nagy, John P. Toscano, Yoshito Kumagai, Catherine F. Henderson, Robert N. Goddu, Joseph Lin and Jon M. Fukuto

The Reaction of Hydrogen Sulfide with Disulfides: Formation of a Stable Trisulfide and Implications to Biological Systems

British Journal of Pharmacology (2019) 176, 671-683.
PubMed Link

Viktor Kozich, Tamás Ditrói, Jitka Sokolová, Michaela Křížková, Jakub Krijt, Pavel Ješina, Péter Nagy*

Metabolism of sulfur compounds in homocystinurias

British Journal of Pharmacology (2019) 176, 594-606.
PubMed Link

Anke Wind, Joris van Dijk, Isabelle Nefkens, Wineke van Lent, Péter Nagy, Ernestas Janulionis, Tuula Helander, Francisco Rocha-Goncalves and Wim van Harten

Development of a benchmark tool for cancer centers; results from a pilot exercise

BMC Health Services Research (2018) 18:764.
PubMed Link

Jon M. Fukuto, Louis J. Ignarro, Péter Nagy, David A. Wink, Christopher G. Kevil, Martin Feelisch, Miriam M. Cortese‐Krott, Christopher L. Bianco, Yoshito Kumagai, Adrian J. Hobbs, Joseph Lin, Tomoaki Ida, Takaaki Akaike

Biological Hydropersulfides and Related Polysulfides: A New Concept and Perspective in Redox Biology

FEBS Letters (2018) 592(12), 2140-2152
PubMed Link

László Potor, Péter Nagy, Gábor Méhes, Zoltán Hendrik, Viktória Jeney, Dávid Pethő, Anita Vasas, Zoltán Pálinkás, Enikő Balogh, Ágnes Gyetvai, Matthew Whiteman, Roberta Torregrossa, Mark E. Wood, Sándor Olvasztó, Péter Nagy, György Balla, József Balla

Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction In Atherosclerotic Lesion

Oxidative Medicine and Cellular Longevity (2018) Volume 2018
PubMed Link

David E. Heppner, Milena Hristova, Tomoaki Ida, Ana Mijuskovic, Christopher M. Dustin,  Virág Bogdándi, Jon M. Fukuto, Tobias P. Dick, Péter Nagy, Jianing Li, Takaaki Akaike, Albertvan der Vliet

Cysteine perthiosulfenic acid (Cys-SSOH): A novel intermediate in thiol-based redox signaling?

Redox Biology (2018) 14, 379-385.
PubMed Link

Gábor Rubovszky, Barna Budai, Erna Ganofszky, Zsolt Horváth, Éva Juhos, Balázs Madaras ,Tünde Nagy, Eszter Szabó, Tamás Pintér, Erika Tóth, Péter Nagy, István Láng, Erika Hitre

Predictive Value of Early Skin Rash in Cetuximab-Based Therapy of Advanced Biliary Tract Cancer

Pathology & Oncology Research (2018) 24(2), 237-244.
PubMed Link

Dorottya Garai, Zoltán Pálinkás, József Balla, Anthony J. Kettle, Péter Nagy*

Measurements for sulfide-mediated inhibition of myeloperoxidase activity

In: Bełtowski J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY (2019) 179-203.

Éva Dóka, Elias S. J. Arnér, Edward E. Schmidt, Péter Nagy*

ProPerDP, a Protein Persulfide Detection Protocol

In: Bełtowski J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY (2019) 51-77.

 

Christopher Kevil, Miriam M. Cortese-Krott, Péter Nagy, Martin Feelisch, Csaba Szabó

Cooperative interactions between NO and H2S: chemistry, biology, physiology, pathophysiology 

Nitric Oxide Biology and Pathobiology 3rd Edition Ignarro L.J., Ed. Elsevier: (2017) 57-83 Invited chapter.

Dorottya Garai, Bessie B. Ríos-González, Paul G. Furtmüller, Jon M. Fukuto, Ming Xian,

Juan López-Garriga, Christian C. Obinger, Péter Nagy*

Mechanisms of myeloperoxidase catalyzed oxidation of H2S by H2O2 or O2 to produce potent protein Cys-polysulfide-inducing species

Free Radical Biology and Medicine (2017) 113, 551–563.
PubMed Link

Takaaki Akaike, Tomoaki Ida, Fan-Yan Wei, Motohiro Nishida, Yoshito Kumagai, Md. Morshedul Alam, Hideshi Ihara, Tomohiro Sawa, Tetsuro Matsunaga1, Shingo Kasamatsu, Akiyuki Nishimura, Masanobu Morita, Kazuhito Tomizawa, Akira Nishimura, Satoshi Watanabe, Kenji Inaba, Hiroshi Shima, Nobuhiro Tanuma, Minkyung Jung, Shigemoto Fujii, Yasuo Watanabe, Masaki Ohmuraya, Péter Nagy, Martin Feelisch, Jon M. Fukuto & Hozumi Motohashi

Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics

Nature Communications (2017) 8(1), 1177
PubMed Link

Miriam Margherita Cortese-Krott, Anne Koning, Gunter Georg Kuhnle, Péter Nagy, Christopher Bianco, Andreas Pasch, David A Wink, Jon Fukuto, Alan A Jackson, Harry van Goor, Kenneth R Olson, Martin Feelisch

The Reactive Species Interactome: Evolutionary Emergence, Biological Significance, and Opportunities for Redox Metabolomics and Personalized Medicine

Antioxidants and Redox Signaling (2017) 27(10), 684-712.
PubMed Link

Bartosz Szczesny, Michela Marcatti, John R. Zatarain, Nadiya Druzhyna, John E. Wiktorowicz, Péter Nagy, Mark R. Hellmich & Csaba Szabó

Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics

Scientific Reports (2016) 6, 36125.
PubMed Link

Gábor Sirokmány, Anna Pató, Melinda Zana, Ágnes Donkó, Adrienn Bíró, Péter Nagy, Miklós Geiszt

Epidermal growth factor-induced hydrogen peroxide production is mediated by dual oxidase 1

Free Radical Biology and Medicine (2016) 97, 204-211
PubMed Link

Éva Dóka, Irina Pader, Adrienn Bíró, Katarina Johansson, Qing Cheng, Krisztina Ballagó, Justin R. Prigge, Daniel Pastor-Flores, Tobias P. Dick, Edward E. Schmidt, Elias S. J. Arnér and Péter Nagy*

Novel persulfide detection method reveals protein persulfide and polysulfide reducing functions of thioredoxin- and glutathione-systems

Science Advances (2016) 2(1):e1500968.
PubMed Link

Tamás Baranyai, Kata Herczeg, Zsófia Onódi, István Voszka, Károly Módos, Nikolett Marton, György Nagy, Imre Mäger, Matthew J Wood, Samir El Andaloussi, Zoltán Pálinkás, Vikas Kumar, Péter Nagy, Ágnes Kittel, Edit Irén Buzás, Péter Ferdinandy, Zoltán Giricz

Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods

PLoS One (2015) 10(12):e0145686.
PubMed Link

Miriam M Cortese-Krott, Gunter GC Kuhnle, Alex Dyson, Bernadette O Fernandez, Marian Grman, Jenna F DuMond, Mark P Barrow, George McLeod, Hidehiko Nakagawa, Karol Ondrias, Péter Nagy, S Bruce King, Joseph E Saavedra, Larry K Keefer, Mervyn Singer, Malte Kelm, Anthony R Butler, Martin Feelisch

Key bioactive reaction products of the NO/H2S interaction are S/N-hybrid species, polysulfides, and nitroxyl

Proceedings of the National Academy of Sciences (2015) 112(34):E4651-60.
PubMed Link

Andrea Berenyiova, Marian Grman, Ana Mijuskovic, Andrej Stasko, Anton Misak, Péter Nagy, Elena Ondriasova, Sona Cacanyiova, Vlasta Brezova, Martin Feelisch, Karol Ondrias

The reaction products of sulfide and S-nitrosoglutathione are potent vasorelaxants

Nitric Oxide (2015) 46:123-30.
PubMed Link

Anita Vasas, Éva Dóka, István Fábián, Péter Nagy*

Kinetic and thermodynamic studies on the disulfide-bond reducing potential of hydrogen sulfide

Nitric Oxide (2015) 46:93-101.
PubMed Link

Zoltán Pálinkás, Paul G Furtmüller, Attila Nagy, Christa Jakopitsch, Katharina F Pirker, Marcin Magierowski, Katarzyna Jasnos, John L Wallace, Christian Obinger, Péter Nagy*

Interactions of hydrogen sulfide with myeloperoxidase

British Journal of Pharmacology (2015) 172(6):1516-32.
PubMed Link

David Peralta, Agnieszka K Bronowska, Bruce Morgan, Éva Dóka, Koen Van Laer, Péter Nagy, Frauke Gräter, Tobias P Dick

A proton relay enhances H2O2 sensitivity of GAPDH to facilitate metabolic adaptation

Nature Chemical Biology (2015) 11(2):156-63.
PubMed Link

Péter Nagy*

Mechanistic chemical perspective of hydrogen sulfide signaling

Methods in Enzymology (2015) 554:3-29.
PubMed Link

J Xu, SE Eriksson, M Cebula, T Sandalova, E Hedström, I Pader, Q Cheng, CR Myers, WE Antholine, P Nagy, Ulf Hellman, G Selivanova, Y Lindqvist, ESJ Arnér

The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death

Cell Death & Disease (2015) 6:e1616.
PubMed Link

Invited lectures

Speaker: Prof. Péter Nagy

 

2020  Invited speaker at the Gordon Research Conference on Thiol-Based Redox Regulation & Signaling, Castelldefels, Spain

2019  Invited speaker at the Persulfide Conference 2019, Sendai, Japan

2019  Control of Protein Function Through Oxidation and Reduction of Persulfidated States

          Invited speaker at the Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan

2019  Redox-tumorbiology; an emerging field in cancer research

          Speaker at the Regional Conference on Partnership and Cooperation in Oncology, Budapest, Hungary

2018  Speciation of reactive sulfur species: Do we have any clue about what’s inside the cell?

          Invited Speaker at the 4th meeting of the study group for redox biology of the German Society for Molecular Biology and Biochemistry, Berlin, Germany

2018  Speciation of reactive sulfur species: do we have any clue about what’s inside the cell?

          Invited speaker at the Thiol-Based Redox Regulation and Signaling (Gordon Research Conference), Spain

2018  Reactive sulfur species: mechanistic considerations for their regulatory functions in redox biology

          Invited Speaker and session chair/organizer of the Redox Biology section at the 2018 FEBS conference, Prague, Czech Republic

2018  Dynamic redox cycling of hydrogen sulfide and polysulfide species could represent an important regulatory element in sulfur biology

          Invited Speaker and session chair/organizer at the 2018 SFRRI, Lisbon, Portugal 

2018  Speciation of reactive sulfur species: do we have any clue about what’s inside the cell?

          Invited Speaker and member of the scientific advisory board at the 5th World Congress on Hydrogen Sulfide in Biology and Medicine, Toronto, Canada, May 31-June 3

2017  Dynamic redox cycling of hydrogen sulfide and polysulfide species could represent an important regulatory element in sulfur biology

          Invited Speaker organizer of the Plant and Human Sulfur Biology Conference 2017, Balatonfüred, Hungary

2017  Roles of the thioredoxin and glutathione systems in reduction of inorganic- and Cys-polysulfide species

          Invited Speaker at the SE2017: The 11th International Symposium on Selenium in Biology and Medicine and The 5th International Conference on Selenium in the environment and Human health, Stockholm, Sweden

2017  Hydrogen Sulfide Signaling

          Invited Lecture at the Redox regulation, oxidative stress and selenoproteins - Summer Graduate Course, Karolinska Institute, Stockholm, Sweden

2017  Molecular models of hydrogen sulfide-mediated protection against oxidative stress

Invited Speaker at 90th Annual Meeting of Japanese Society for Bacteriology, Sendai, Japan

2016  Some aspects of sulfur biology from a mechanistic chemist's perspective

Invited Seminar at the Center for Molecular Medicine Cologne University, Cologne, Germany

2016  Chemical aspects of sulfane sulfur biology

Invited Speaker at the Tohoku University Graduate School of Medicine, Sendai, Japan 

2016  Advances and challenges in the field of H2S biology

Invited Speaker at the Dojindo Inc. HQs in Kumamoto, Japan

2016  Bio-chemical aspects of thiol oxidation

Invited Seminar Kyoto University, Kyoto, Japan

2016  Molecular pathways in persulfide biology

Invited Speaker at the 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide held jointly with the 16th Annual Scientific Meeting of the Nitric Oxide Society of Japan, Sendai, Japan

2016  Hydrogen sulfide, the new kid on the block in redox signaling

Invited Speaker at the Society for Free Radical Research-Europe, Budapest, Hungary

2016  Insights into the molecular pathways of persulfide-mediated redox signaling

Invited Speaker at the 4th International Conference on the Biology of Hydrogen Sulfide, Napoli, Italy

2015  Protein persulfides: Insights into the molecular mechanisms of H2S signaling

Invited plenary lecture at the Joint Meeting of the Societies for Free Radical Research Australasia and Japan, Christchurch, New Zealand

2015  Mechanistic chemical perspective of thiol redox biology

Invited Speaker at the Thiol-based redox switches in life sciences ESF-EMBO conference, San Feliu de Guixols, Spain

2015  Superoxide-mediated post-translational modification of tyrosine residues

Invited Speaker at the Society for Free Radical Research- Europe meeting Stuttgart, Germany

2015  Hydrogen sulfide and redox signaling

Invited Speaker at the “Redox Regulation, Oxidative Stress, and Selenoproteins.” Medical University of South Carolina in Charleston, S.C., USA

2015  Mechanistic Chemical Perspective of Hydrogen Sulfide Signaling

Invited Speaker at the 3rd European Conference on the Biology of Hydrogen Sulfide, Athens, Greece

2015  Mechanistic Chemical Perspective of Hydrogen Sulfide Signaling

Invited Speaker at the „RISE Enhancing Biomedical Sciences and Biomedical Engineering in Science and Technology”Mayagüez, Puerto Rico

2015  Redox biochemistry of thiol proteins and hydrogen sulfide

Invited Seminar LSU Health Shreveport, LA, USA

Funding

  • 2019 NKFIH Thematic Excellence Program, Consortial Research Program entitled “Innovations in Breast Cancer Care”                           PI: Prof. Péter Nagy
  • 2018-2022 NKFIH K129286, „Protecting roles of hydrogen sulfide in MPO-ANCA associated vasculitis” PI: Prof.Péter Nagy
  • 2017-2019 NKFIH KH126766 (Funding for research groups with internationally outstanding high impact results), „Cooperative actions of Trx/GSH systems and the CARS2 system in mediating protein polysulfidation” PI: Prof.Péter Nagy
  • 2013-2017 OTKA K109843, „Peroxidase function of peroxiredoxins in relation with cancer” PI: Prof. Péter Nagy
  • 2016-2018 NIH 1R21AG055022-01, ’Redox regulation in the hepatostat’  PI: Prof. Edward E. Schmidt, Montana State University; co-PIs: Prof. Elias S.J. Arnér, Prof.Péter Nagy

Funding

 

  • 2019 NKFIH Thematic Excellence Program, Consortial Research Program entitled “Innovations in Breast Cancer Care”                           PI: Prof. Péter Nagy2018-2022 NKFIH K129286, „Protecting roles of hydrogen sulfide in MPO-ANCA associated vasculitis”                                                             PI: Prof.Péter Nagy
  • 2017-2019 NKFIH KH126766 (Funding for research groups with internationally outstanding high impact results), „Cooperative actions of Trx/GSH systems and the CARS2 system in mediating protein polysulfidation”                                    PI: Prof.Péter Nagy
  • 2013-2017 OTKA K109843, „Peroxidase function of peroxiredoxins in relation with cancer”

PI: Prof. Péter Nagy

  • 2016-2018 NIH 1R21AG055022-01, ’Redox regulation in the hepatostat’         
     PI: Prof. Edward E. Schmidt, Montana State University; co-PIs: Prof. Elias S.J. Arnér, Prof.Péter Nagy

 

Teaching

  1. Graduate and undergraduate course:
    • “Redox chemistry in biological systems (lecture in Hungarian)” 
    • “Redox biochemistry. Formation of Reactive Oxygen Species (ROS) and their biological functions. Prof. Péter Nagy, Dr. Éva Dóka, Tamás Ditrói, University of Debrecen, Institute of Chemistry
    • „Redox Biochemistry” (lecture in Hungarian) Prof. Péter Nagy, Dr. Éva Dóka, Tamás Ditrói and Dr. Katalin Erdélyi at Semmelweis University.
      1. PhD students 
    • “Roles of Hydrogen Sulfide and its Metabolites in Neutrophil Function and Redox Signaling.”          

Student: Anita Varga-Vasas – Successful degree (Summa Cum Laude)

Advisor: Prof. Péter Nagy      

University of Debrecen, Doctoral School of Chemistry

  • „ Mechanistic biochemical investigations of Hydrogen sulfide signaling” 

Student: Virág Bogdándi – thesis submitted for review

Advisor:  Prof. Péter Nagy 

University of Pécs, PhD program in Interdisciplinary Medical Sciences

  • „ Roles of hydrogen sulfide in neutrophil-mediated inflammatory processes.” 

Student: Dorottya Garai – ongoing (3rd year)

Advisor: Prof. Péter Nagy

University of Debrecen, Kálmán Laki Doctoral School of Biomedical and Clinical Sciences

  1. .Prof. Péter Nagy – visiting professor at Semmelweis University, Budapest, Hungary
  2. International Chemistry Olympiad (IChO) preparatory course (2014 – 2019)

Éva Dóka – Instructor in Reaction kinetics – Institute of Chemistry, ELTE University

 

Main collaborators

  • Prof. Takaaki Akaike, Tohoku University Graduate School of Medicine, Sendai, Japan
  • Prof. Elias Arnér, Karolinska Institutet, Stockholm, Sweden
  • Prof. József Balla, University of Debreceni Egyetem, Dept. of Nephrology
  • Prof. Tobias Dick DKFZ, Heidelberg, Germany
  • Prof. Miklós Geiszt, Semmelweis University, Dept. of Physiology
  • Prof. Martin Feelisch, Southampton University, Southampton, United Kingdom 
  • Prof. Jon Fukuto, Department of Chemistry, Sonoma State University, Sonoma, California, USA
  • Prof. Viktor Kožich, General University Hospital in Prague, Czech Republic
  • Prof. Janne Lehtiö, Dept. of Onco-Pathology and Proteomics facility at the SciLifeLab, Stockholm, Sweden
  • Prof. Juan Lopez Garriga, Recinto Universitario de Mayagüez, Mayagüez, Puerto Rico
  • Prof. Ed Schmidt, Dept. of Microbiology and Immunology, Montana State University, Montana, USA
  • Prof. John L. Wallace, Department of Physiology and Pharmacology, University of Calgary, Calgary, Canada; Antibe Therapeutics, Toronto, Canada
  • Prof. Christine Winterbourn, University of Otago Christchurch, Centre for Free Radical Research, Christchurch, New-Zealand

 

Methods and equipment

Metabolomics and proteomics platform

Enzymatic activity, analysis of various metabolites and reactive sulfur species by mass spectrometry and liquid chromatography

  • Thermo LTQ XL mass spectrometer
  • EASY-nLC II nano HPLC
  • ·         Thermo Scientific UltiMate 3000 HPLC with fluorescent detector

Cell and tissue culture 

Investigation of human cancer cell lines – breast, lung, pancreas, melanoma 

Development of xenograft models in immune deficient mice

Examination of neutrophil granulocytes isolated from human blood plasma

Processsing of mouse tissue samples for metabolomics and proteomics

  • sterile cell culture hoods, incubator, hypoxic chamber, microscope, etc. 
  • tissue staining procedures (immunohistochemistry)
  • ·         Braun Mikro-Dismembrator II tissue homogenizator

Molecular biology

Gene silencing with lentiviral and CRISPR techniques

Transient and stable transfection of mammalian cell lines

Design and amplification of plasmid constructs

  • rotary shaker suitable for bacterial culture 
  • PCR instrument
  • ·         agarose gel electrophoresis equipment for DNA/RNA separation

SDS-PAGE and Western blot

immunoblotting of purified proteins and whole cell/tissue lysates

2D és 2D diagonal gel electrophoresis

  • CBS Scientific gel electrophoresis equipment
  • BioRad Mini-Protean wet Western blot equipment
  • 2D gel electrophoresis equipment
  • Syngene Gbox gel documentation system
  • ·         Gel staining methods: Coomassie Brilliant Blue, Ponceau S, silver staining, fluorescent staining

Enzyme kinetics

Activity assays, UV-Vis spectrophometry, solution phase kinetics and mechanisms

  • BioTek PowerWave XS spectrophotometer and microplate reader
  • Agilent Cary 8454 UV-Vis photodiode array (PDA) spectrophotometer
  • Tecan Spark 10M multimode microplate reader – fluorescence, absorbance and luminescence modules; suitable to maintain living cell cultures

 

Methods of in-lab or collaborative development 

  • ProPerDP – Protein Persulfide Detection Protocol

Experimental procedure for protein persulfide detection from purified proteins, intact cells and tissue samples

Doka et al., Science Advances (2016) 2(1):e1500968.  PubMed Link

Doka et al., Methods Mol Biol..

  • Quantitative LC-MS detection of low molecular weight and protein derived per/polysulfide species and reactive sulfur species (HPE-IAM labeling and suitable digestion)

Hamid et al., Redox Biology (2019) 21, 101096.  PubMed Link

Akaike et al., Nature Communications (2017) 8(1), 1177  PubMed Link

  • Quantitative determination of H2S from biological matrices by monobromobimane labeling 

Ditroi et al., (2019) 136, 146-158.  PubMed Link

  • Metabolomics 

High sensitivity LC-MS detection of transsulfuration metabolytes

  • CBS (cystathionine beta synthase) enzymatic activity from blood plasma 

Krijt et al., Journal of Inherited Metabolic Disease, (2011) 34(1):49-55.  PubMed Link

  • Fluorescence based MPO peroxidase activity assay from supernatant of primed neutrophils

Palinkas et al., British Journal of Pharmacology (2015) 172(6):1516-32.  PubMed Link

Garai et al., Methods in Molecular Biology Beltowski J, editor Springer, in press; 2017.

  • Polysulfide generation activity of MPO by customized fluorescent assay

Garai et al., Free Radical Biology and Medicine (2017) 113, 551–563.  PubMed Link

 

 

Links

  • ·         6th World Congress on Hydrogen Sulfide in Biology & Medicine 

http://h2s2020.hu/

+36 (1) 224 8600
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