HEAD OF THE DEPARTMENT
Department of Molecular Immunology and Toxicology (MITO)
Our group mainly focuses on research projects, although we routinely carry out measurements for diagnostic purposes.
• We determine the concentration of methotrexat (MTX) from blood plasma samples of cancer patients in order to predict potential side effects and for monitoring the pharmacokinetics of the drug.
• New experimental methods for the determination of various tumor and other biological markers are currently under development in our laboratory. Following careful validation, we are planning to make these techniques available for diagnostics and clinical care.
Research Advisory Board
• Internal Research Committee
o Scientific Director
o Director of Innovation and Development
o Deputy Director General
o Clinical Director
o Director of Center of Surgery
o Director of Medical Oncology
o Director of Diagnostics and Imaging
o Director of the Center of Pathology
• External Research Advisory Board
o Professor Peter Boyle, Director of iPRI
o Professor Gunnar Saeter, Director of Research, Oslo University Hospital
o Professor Ulrik Ringborg, Director Cancer Center, Karolinska Institutet
Research Departments at the National Institute of Oncology
• Molecular Genetics
• Molecular Immunology and Toxicology
• Experimental Pharmacology
• Radiation Biology
• Clinical Research
Current Research Programmes at the National Institute of Oncology
• Analysis of genetic diversity in the Hungarian population
• Discovering new targets to develop novel therapies
• Elucidation of molecular pathways responsible for treatment resistance
• Identification of new molecular markers for diagnosis and response to therapy
• Molecular diagnostics facility to ensure precision medicine to become a success. (molecular diagnostics, imaging, novel genetic based diagnostic panels, functional proteomics, innovative biomarkers, analyses of circulating tumor cells or exosomes etc)
• Prevention and early detection of hereditary cancers (breast, colorectal)
• Wide scale of high profile research activities
o Both prospective and retrospective studies
o Increasing number of clinical trials and patients enrolled in these trials
In 2015: 93 with 894 newly enrolled patients
In 2016: 111 with 1062 newly enrolled patients
o Sponsored trials
o Collaborations with leading agencies (EORTC, International Breast Cancer Study Group etc)
o Investigator initiated and academic trials
• New basic research groups
o Department of Molecular Immunology and Toxicology
o Experimental Pharmacology
• New directions in basic cancer research
o Functional Proteomics
o Redox tumor-biology
o Development of novel therapies
o Mechanistic insights into regulation of cellular signaling from new angles
o Drug-peptide conjugates
National Oncogenomic project
• Coordinating role for NIO
• In the framework of this project—pioneering in the Eastern European context—the following objectives are aimed to be achieved by the consortium:
• Genomic examinations of solid organ and hematopoietic tumors in Hungarian patients.
• Identification of "driver" genes responsible for tumor phenotypes and the associated signaling pathways.
• Classification of the tumors’ new molecular genetic alterations using Big Data analyses.
• Detection of specific genetic alterations of individual patients.
National Excellence Program
• Innovations in breast cancer care
The ultimate goal of the project is to develop and implement a precision medicine model for the treatment of breast cancer by harmonizing surgery, radiotherapy, medical oncology, imaging, molecular diagnostics and basic research endeavors.
Wide scale of platforms to bridge clinical and basic research activities
o Seminar series
o Journal clubs
• Increasing number of collaborative projects and publications
o For example: Development of novel functional proteomics techniques to study resistance mechanisms for targeted therapies
• Risk estimation of smoking and drinking patients
• Hereditary risk of cancer in breast, ovarian, colon and prostate cancer patients
• Identification of gene polymorphisms in breast, colorectal, head and neck and prostate cancers
Research Infrastructure at the National Institute of Oncology
• Designated Research Buildings
• Core Facilities
o Next Generation Sequencing:
Roche FLX Genome Sequencer System, Illumina MiSeq System,
o Mass spectrometry: Thermo Orbitrap Fusion and LTQ XL mass spects
o Confocal microscope: NIKON Eclipse 80i C1 confocal microscope
o Flow cytometer: Beckman Coulter Cell Lab Quanta SC.
o DNA chip and high throughput protein expression profile analyzer
o Research Support Services
o Biobank (new centralized system development nearly finished)
o Animal House at Special Pathogen Free level:
3000 live animal
• 5000 used annually
• 1/3 of the animals are immune suppressed
• Over 50 different tumor cell lines with 6-8 different inoculation sites
o Mechanical and electronics facility
o Isotope laboratory
o Research supplies warehouse
o Designated unit at the finances department
Research Output at the National Institute of Oncology
The number of annually published peer-reviewed articles, books, book chapters is >125 with a cumulative annual impact factor of > 600 in 2017 and 2018.
The Department of Molecular Immunology and Toxicology was founded in 2011 and introduced a new point of view within the research platform of the National Institute of Oncology. The main motivation of our studies is to investigate the role of free radicals and reactive oxidizing/reducing species in tumor biology. Reactive Oxygen Species (ROS) and Reactive Sulfur Species (RSS) are standing in the focus point of our efforts, with special regards to their function in oxidative stress (e.g. chemo- or radiation therapy) and redox signaling (identification of therapeutic targets and unraveling of drug resistance pathways).
Targeted therapeutical approaches revolutionized the care of cancer patients, in a sense that unlike traditional treatments, they are designed to specifically eliminate tumor cells, exploiting their molecular fingerprints. According to a widely accepted perception, post-translational modifications, originating from protein-protein interactions, alter the functions of proteins thus affecting onco-signaling of cancer cells. Beside the thoroughly examined phosphorylation cascades, oxidation induced modifications are emerging participants of the regulation of signaling pathways.
Malignant cells overexpress ROS and RSS species compared to their healthy counterparts. Increased ROS and RSS flux suggest that anomalies in redox regulated signaling processes is a hallmark feature of tumor biology, providing potential therapeutic targets. This innovative approach may gain special importance for cancer types with no existing targeted therapy. In the framework of our research, we make attempts to facilitate the treatments of these severe conditions, by better understanding the molecular background of the underlying biology.
Our research group consists of experienced chemists, biologists and biochemists, collaborating on multidisciplinary projects on a daily basis.
• Prof. Peter Nagy, DSc.
Head of department
(1)-224-8600 / Extension 1170 or 3644
• Zsuzsanna Lénárt
(1)-224-8600 / 2170
• Dr. Katalin Erdélyi
• Dr. Beatrix Kotlán
• Dr. Éva Dóka
• Tamás Ditrói
• Ágnes Czikora biologist
• Dorottya Garai
biologist, PhD student
• Klaudia Galambos
MSc student of biology, ELTE university
• Noémi Balog
leading laboratory assistant
(1)-224-8600 / 1180
• Attila Nagy
chemistry laboratory technician
* corresponding author
Tamás Ditrói, Attila Nagy, Diego Martinelli, András Rosta, Viktor Kožich, Péter Nagy*
Comprehensive analysis of how experimental parameters affect H2S measurements by the monobromobimane method
Free Radical Biology and Medicine (2019) 136, 146-158.
John L. Wallace, Péter Nagy, Troy D. Feener, Thibault Allain, Tamás Ditrói, David J. Vaughan, Marcelo N. Muscara, Gilberto de Nucci & Andre G. Buret
A Proof-of-Concept, Phase 2 Clinical Trial of the Gastrointestinal Safety of a Hydrogen Sulfide-Releasing Anti-Inflammatory Drug
British Journal of Pharmacology (2019) Epub ahead of print
Katalin Éva Sikura, Tamás Szerafin, László Potor, Melinda Oros, Péter Nagy, Gábor Méhes, Zoltán Hendrik, Abolfazl Zarjou, Anupam Agarwal, Niké Posta, Roberta Torregrossa, Matthew Whiteman, György Balla, József Balla
Hydrogen sulfide abrogate heart valve calcification: implications for calcific aortic valve disease
British Journal of Pharmacology (2019) Epub ahead of print
Joseph Lin, Masahiro Akiyama, Iris Bica, Faith T. Long, Catherine F. Henderson, Robert N. Goddu, Valeria Suarez, Blaine Baker, Tomoaki Ida, Yasuhiro Shinkai, Péter Nagy, Takaaki Akaike, Jon M. Fukuto, and Yoshito Kumagai
The Uptake and Release of Polysulfur Cysteine Species by Cells: Physiological and Toxicological Implications
Chemical Research in Toxicology (2019) 32, 447-455.
Hisyam Abdul Hamid, Akira Tanaka, Tomoaki Ida, Akira Nishimura, Tetsuro Matsunaga, Shigemoto Fujii, Masanobu Morita, Tomohiro Sawa, Jon M. Fukuto, Péter Nagy, Ryouhei Tsutsumi, Hozumi Motohashi, Hideshi Ihara, and Takaaki Akaike
Polysulfide stabilization by tyrosine and hydroxyphenyl-containing derivatives that is important for a reactive sulfur metabolomics analysis
Redox Biology (2019) 21, 101096.
Péter Nagy*, Günter Schwarz and Stanislav Kopriva
Highlighted mechanistic aspects in the chemical biology of reactive sulfur species
British Journal of Pharmacology (2019) 176, 511-513.
Virág Bogdándi, Tomoaki Ida, Thomas R Sutton, Christopher Bianco, Tamás Ditrói, Grielof Koster, Hillary A Henthorn, Magda Minnion, John P Toscano, Albert van der Vliet, Michael D Pluth, Martin Feelisch, Jon M Fukuto, Takaaki Akaike and Péter Nagy*
Speciation of Reactive Sulfur Species and their Reactions with Alkylating Agents: Do we have any clue about what is present inside the cell?
British Journal of Pharmacology (2019) 176, 646-670.
Christopher L. Bianco, Takaaki Akaike, Tomoaki Ida, Péter Nagy, John P. Toscano, Yoshito Kumagai, Catherine F. Henderson, Robert N. Goddu, Joseph Lin and Jon M. Fukuto
The Reaction of Hydrogen Sulfide with Disulfides: Formation of a Stable Trisulfide and Implications to Biological Systems
British Journal of Pharmacology (2019) 176, 671-683.
Viktor Kozich, Tamás Ditrói, Jitka Sokolová, Michaela Křížková, Jakub Krijt, Pavel Ješina, Péter Nagy*
Metabolism of sulfur compounds in homocystinurias
British Journal of Pharmacology (2019) 176, 594-606.
Anke Wind, Joris van Dijk, Isabelle Nefkens, Wineke van Lent, Péter Nagy, Ernestas Janulionis, Tuula Helander, Francisco Rocha-Goncalves and Wim van Harten
Development of a benchmark tool for cancer centers; results from a pilot exercise
BMC Health Services Research (2018) 18:764.
Jon M. Fukuto, Louis J. Ignarro, Péter Nagy, David A. Wink, Christopher G. Kevil, Martin Feelisch, Miriam M. Cortese‐Krott, Christopher L. Bianco, Yoshito Kumagai, Adrian J. Hobbs, Joseph Lin, Tomoaki Ida, Takaaki Akaike
Biological Hydropersulfides and Related Polysulfides: A New Concept and Perspective in Redox Biology
FEBS Letters (2018) 592(12), 2140-2152
László Potor, Péter Nagy, Gábor Méhes, Zoltán Hendrik, Viktória Jeney, Dávid Pethő, Anita Vasas, Zoltán Pálinkás, Enikő Balogh, Ágnes Gyetvai, Matthew Whiteman, Roberta Torregrossa, Mark E. Wood, Sándor Olvasztó, Péter Nagy, György Balla, József Balla
Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction In Atherosclerotic Lesion
Oxidative Medicine and Cellular Longevity (2018) Volume 2018
David E. Heppner, Milena Hristova, Tomoaki Ida, Ana Mijuskovic, Christopher M. Dustin, Virág Bogdándi, Jon M. Fukuto, Tobias P. Dick, Péter Nagy, Jianing Li, Takaaki Akaike, Albertvan der Vliet
Cysteine perthiosulfenic acid (Cys-SSOH): A novel intermediate in thiol-based redox signaling?
Redox Biology (2018) 14, 379-385.
Gábor Rubovszky, Barna Budai, Erna Ganofszky, Zsolt Horváth, Éva Juhos, Balázs Madaras ,Tünde Nagy, Eszter Szabó, Tamás Pintér, Erika Tóth, Péter Nagy, István Láng, Erika Hitre
Predictive Value of Early Skin Rash in Cetuximab-Based Therapy of Advanced Biliary Tract Cancer
Pathology & Oncology Research (2018) 24(2), 237-244.
Dorottya Garai, Zoltán Pálinkás, József Balla, Anthony J. Kettle, Péter Nagy*
Measurements for sulfide-mediated inhibition of myeloperoxidase activity
In: Bełtowski J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY (2019) 179-203.
Éva Dóka, Elias S. J. Arnér, Edward E. Schmidt, Péter Nagy*.
ProPerDP, a Protein Persulfide Detection Protocol
In: Bełtowski J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY (2019) 51-77.
Christopher Kevil, Miriam M. Cortese-Krott, Péter Nagy, Martin Feelisch, Csaba Szabó
Cooperative interactions between NO and H2S: chemistry, biology, physiology, pathophysiology
Nitric Oxide Biology and Pathobiology 3rd Edition Ignarro L.J., Ed. Elsevier: (2017) 57-83 Invited chapter.
Dorottya Garai, Bessie B. Ríos-González, Paul G. Furtmüller, Jon M. Fukuto, Ming Xian,
Juan López-Garriga, Christian C. Obinger, Péter Nagy*
Mechanisms of myeloperoxidase catalyzed oxidation of H2S by H2O2 or O2 to produce potent protein Cys-polysulfide-inducing species
Free Radical Biology and Medicine (2017) 113, 551–563.
Takaaki Akaike, Tomoaki Ida, Fan-Yan Wei, Motohiro Nishida, Yoshito Kumagai, Md. Morshedul Alam, Hideshi Ihara, Tomohiro Sawa, Tetsuro Matsunaga1, Shingo Kasamatsu, Akiyuki Nishimura, Masanobu Morita, Kazuhito Tomizawa, Akira Nishimura, Satoshi Watanabe, Kenji Inaba, Hiroshi Shima, Nobuhiro Tanuma, Minkyung Jung, Shigemoto Fujii, Yasuo Watanabe, Masaki Ohmuraya, Péter Nagy, Martin Feelisch, Jon M. Fukuto & Hozumi Motohashi
Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics
Nature Communications (2017) 8(1), 1177
Miriam Margherita Cortese-Krott, Anne Koning, Gunter Georg Kuhnle, Péter Nagy, Christopher Bianco, Andreas Pasch, David A Wink, Jon Fukuto, Alan A Jackson, Harry van Goor, Kenneth R Olson, Martin Feelisch
The Reactive Species Interactome: Evolutionary Emergence, Biological Significance, and Opportunities for Redox Metabolomics and Personalized Medicine
Antioxidants and Redox Signaling (2017) 27(10), 684-712.
Bartosz Szczesny, Michela Marcatti, John R. Zatarain, Nadiya Druzhyna, John E. Wiktorowicz, Péter Nagy, Mark R. Hellmich & Csaba Szabó
Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics
Scientific Reports (2016) 6, 36125.
Gábor Sirokmány, Anna Pató, Melinda Zana, Ágnes Donkó, Adrienn Bíró, Péter Nagy, Miklós Geiszt
Epidermal growth factor-induced hydrogen peroxide production is mediated by dual oxidase 1
Free Radical Biology and Medicine (2016) 97, 204-211
Éva Dóka, Irina Pader, Adrienn Bíró, Katarina Johansson, Qing Cheng, Krisztina Ballagó, Justin R. Prigge, Daniel Pastor-Flores, Tobias P. Dick, Edward E. Schmidt, Elias S. J. Arnér and Péter Nagy*
Novel persulfide detection method reveals protein persulfide and polysulfide reducing functions of thioredoxin- and glutathione-systems
Science Advances (2016) 2(1):e1500968.
Tamás Baranyai, Kata Herczeg, Zsófia Onódi, István Voszka, Károly Módos, Nikolett Marton, György Nagy, Imre Mäger, Matthew J Wood, Samir El Andaloussi, Zoltán Pálinkás, Vikas Kumar, Péter Nagy, Ágnes Kittel, Edit Irén Buzás, Péter Ferdinandy, Zoltán Giricz
Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods
PLoS One (2015) 10(12):e0145686.
Miriam M Cortese-Krott, Gunter GC Kuhnle, Alex Dyson, Bernadette O Fernandez, Marian Grman, Jenna F DuMond, Mark P Barrow, George McLeod, Hidehiko Nakagawa, Karol Ondrias, Péter Nagy, S Bruce King, Joseph E Saavedra, Larry K Keefer, Mervyn Singer, Malte Kelm, Anthony R Butler, Martin Feelisch
Key bioactive reaction products of the NO/H2S interaction are S/N-hybrid species, polysulfides, and nitroxyl
Proceedings of the National Academy of Sciences (2015) 112(34):E4651-60.
Andrea Berenyiova, Marian Grman, Ana Mijuskovic, Andrej Stasko, Anton Misak, Péter Nagy, Elena Ondriasova, Sona Cacanyiova, Vlasta Brezova, Martin Feelisch, Karol Ondrias
The reaction products of sulfide and S-nitrosoglutathione are potent vasorelaxants
Nitric Oxide (2015) 46:123-30.
Anita Vasas, Éva Dóka, István Fábián, Péter Nagy*
Kinetic and thermodynamic studies on the disulfide-bond reducing potential of hydrogen sulfide
Nitric Oxide (2015) 46:93-101.
Zoltán Pálinkás, Paul G Furtmüller, Attila Nagy, Christa Jakopitsch, Katharina F Pirker, Marcin Magierowski, Katarzyna Jasnos, John L Wallace, Christian Obinger, Péter Nagy*
Interactions of hydrogen sulfide with myeloperoxidase
British Journal of Pharmacology (2015) 172(6):1516-32.
David Peralta, Agnieszka K Bronowska, Bruce Morgan, Éva Dóka, Koen Van Laer, Péter Nagy, Frauke Gräter, Tobias P Dick
A proton relay enhances H2O2 sensitivity of GAPDH to facilitate metabolic adaptation
Nature Chemical Biology (2015) 11(2):156-63.
Mechanistic chemical perspective of hydrogen sulfide signaling
Methods in Enzymology (2015) 554:3-29.
J Xu, SE Eriksson, M Cebula, T Sandalova, E Hedström, I Pader, Q Cheng, CR Myers, WE Antholine, P Nagy, Ulf Hellman, G Selivanova, Y Lindqvist, ESJ Arnér
The conserved Trp114 residue of thioredoxin reductase 1 has a redox sensor-like function triggering oligomerization and crosslinking upon oxidative stress related to cell death
Cell Death & Disease (2015) 6:e1616.
Speaker: Prof. Péter Nagy
2020 Invited speaker at the Gordon Research Conference on Thiol-Based Redox Regulation & Signaling, Castelldefels, Spain
2019 Invited speaker at the Persulfide Conference 2019, Sendai, Japan
2019 Control of Protein Function Through Oxidation and Reduction of Persulfidated States
Invited speaker at the Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan
2019 Redox-tumorbiology; an emerging field in cancer research
Speaker at the Regional Conference on Partnership and Cooperation in Oncology, Budapest, Hungary
2018 Speciation of reactive sulfur species: Do we have any clue about what’s inside the cell?
Invited Speaker at the 4th meeting of the study group for redox biology of the German Society for Molecular Biology and Biochemistry, Berlin, Germany
2018 Speciation of reactive sulfur species: do we have any clue about what’s inside the cell?
Invited speaker at the Thiol-Based Redox Regulation and Signaling (Gordon Research Conference), Spain
2018 Reactive sulfur species: mechanistic considerations for their regulatory functions in redox biology
Invited Speaker and session chair/organizer of the Redox Biology section at the 2018 FEBS conference, Prague, Czech Republic
2018 Dynamic redox cycling of hydrogen sulfide and polysulfide species could represent an important regulatory element in sulfur biology
Invited Speaker and session chair/organizer at the 2018 SFRRI, Lisbon, Portugal
2018 Speciation of reactive sulfur species: do we have any clue about what’s inside the cell?
Invited Speaker and member of the scientific advisory board at the 5th World Congress on Hydrogen Sulfide in Biology and Medicine, Toronto, Canada, May 31-June 3
2017 Dynamic redox cycling of hydrogen sulfide and polysulfide species could represent an important regulatory element in sulfur biology
Invited Speaker organizer of the Plant and Human Sulfur Biology Conference 2017, Balatonfüred, Hungary
2017 Roles of the thioredoxin and glutathione systems in reduction of inorganic- and Cys-polysulfide species
Invited Speaker at the SE2017: The 11th International Symposium on Selenium in Biology and Medicine and The 5th International Conference on Selenium in the environment and Human health, Stockholm, Sweden
2017 Hydrogen Sulfide Signaling
Invited Lecture at the Redox regulation, oxidative stress and selenoproteins - Summer Graduate Course, Karolinska Institute, Stockholm, Sweden
2017 Molecular models of hydrogen sulfide-mediated protection against oxidative stress
Invited Speaker at 90th Annual Meeting of Japanese Society for Bacteriology, Sendai, Japan
2016 Some aspects of sulfur biology from a mechanistic chemist's perspective
Invited Seminar at the Center for Molecular Medicine Cologne University, Cologne, Germany
2016 Chemical aspects of sulfane sulfur biology
Invited Speaker at the Tohoku University Graduate School of Medicine, Sendai, Japan
2016 Advances and challenges in the field of H2S biology
Invited Speaker at the Dojindo Inc. HQs in Kumamoto, Japan
2016 Bio-chemical aspects of thiol oxidation
Invited Seminar Kyoto University, Kyoto, Japan
2016 Molecular pathways in persulfide biology
Invited Speaker at the 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide held jointly with the 16th Annual Scientific Meeting of the Nitric Oxide Society of Japan, Sendai, Japan
2016 Hydrogen sulfide, the new kid on the block in redox signaling
Invited Speaker at the Society for Free Radical Research-Europe, Budapest, Hungary
2016 Insights into the molecular pathways of persulfide-mediated redox signaling
Invited Speaker at the 4th International Conference on the Biology of Hydrogen Sulfide, Napoli, Italy
2015 Protein persulfides: Insights into the molecular mechanisms of H2S signaling
Invited plenary lecture at the Joint Meeting of the Societies for Free Radical Research Australasia and Japan, Christchurch, New Zealand
2015 Mechanistic chemical perspective of thiol redox biology
Invited Speaker at the Thiol-based redox switches in life sciences ESF-EMBO conference, San Feliu de Guixols, Spain
2015 Superoxide-mediated post-translational modification of tyrosine residues
Invited Speaker at the Society for Free Radical Research- Europe meeting Stuttgart, Germany
2015 Hydrogen sulfide and redox signaling
Invited Speaker at the “Redox Regulation, Oxidative Stress, and Selenoproteins.” Medical University of South Carolina in Charleston, S.C., USA
2015 Mechanistic Chemical Perspective of Hydrogen Sulfide Signaling
Invited Speaker at the 3rd European Conference on the Biology of Hydrogen Sulfide, Athens, Greece
2015 Mechanistic Chemical Perspective of Hydrogen Sulfide Signaling
Invited Speaker at the „RISE Enhancing Biomedical Sciences and Biomedical Engineering in Science and Technology”Mayagüez, Puerto Rico
2015 Redox biochemistry of thiol proteins and hydrogen sulfide
Invited Seminar LSU Health Shreveport, LA, USA
- 2019 NKFIH Thematic Excellence Program, Consortial Research Program entitled “Innovations in Breast Cancer Care” PI: Prof. Péter Nagy2018-2022 NKFIH K129286, „Protecting roles of hydrogen sulfide in MPO-ANCA associated vasculitis” PI: Prof.Péter Nagy
- 2017-2019 NKFIH KH126766 (Funding for research groups with internationally outstanding high impact results), „Cooperative actions of Trx/GSH systems and the CARS2 system in mediating protein polysulfidation” PI: Prof.Péter Nagy
- 2013-2017 OTKA K109843, „Peroxidase function of peroxiredoxins in relation with cancer”
PI: Prof. Péter Nagy
- 2016-2018 NIH 1R21AG055022-01, ’Redox regulation in the hepatostat’
PI: Prof. Edward E. Schmidt, Montana State University; co-PIs: Prof. Elias S.J. Arnér, Prof.Péter Nagy
- Graduate and undergraduate course:
- “Redox chemistry in biological systems (lecture in Hungarian)”
- “Redox biochemistry. Formation of Reactive Oxygen Species (ROS) and their biological functions. Prof. Péter Nagy, Dr. Éva Dóka, Tamás Ditrói, University of Debrecen, Institute of Chemistry
- „Redox Biochemistry” (lecture in Hungarian) Prof. Péter Nagy, Dr. Éva Dóka, Tamás Ditrói and Dr. Katalin Erdélyi at Semmelweis University.
- PhD students
- “Roles of Hydrogen Sulfide and its Metabolites in Neutrophil Function and Redox Signaling.”
Student: Anita Varga-Vasas – Successful degree (Summa Cum Laude)
Advisor: Prof. Péter Nagy
University of Debrecen, Doctoral School of Chemistry
- „ Mechanistic biochemical investigations of Hydrogen sulfide signaling”
Student: Virág Bogdándi – thesis submitted for review
Advisor: Prof. Péter Nagy
University of Pécs, PhD program in Interdisciplinary Medical Sciences
- „ Roles of hydrogen sulfide in neutrophil-mediated inflammatory processes.”
Student: Dorottya Garai – ongoing (3rd year)
Advisor: Prof. Péter Nagy
University of Debrecen, Kálmán Laki Doctoral School of Biomedical and Clinical Sciences
- .Prof. Péter Nagy – visiting professor at Semmelweis University, Budapest, Hungary
- International Chemistry Olympiad (IChO) preparatory course (2014 – 2019)
Éva Dóka – Instructor in Reaction kinetics – Institute of Chemistry, ELTE University
- Prof. Takaaki Akaike, Tohoku University Graduate School of Medicine, Sendai, Japan
- Prof. Elias Arnér, Karolinska Institutet, Stockholm, Sweden
- Prof. József Balla, University of Debreceni Egyetem, Dept. of Nephrology
- Prof. Tobias Dick DKFZ, Heidelberg, Germany
- Prof. Miklós Geiszt, Semmelweis University, Dept. of Physiology
- Prof. Martin Feelisch, Southampton University, Southampton, United Kingdom
- Prof. Jon Fukuto, Department of Chemistry, Sonoma State University, Sonoma, California, USA
- Prof. Viktor Kožich, General University Hospital in Prague, Czech Republic
- Prof. Janne Lehtiö, Dept. of Onco-Pathology and Proteomics facility at the SciLifeLab, Stockholm, Sweden
- Prof. Juan Lopez Garriga, Recinto Universitario de Mayagüez, Mayagüez, Puerto Rico
- Prof. Ed Schmidt, Dept. of Microbiology and Immunology, Montana State University, Montana, USA
- Prof. John L. Wallace, Department of Physiology and Pharmacology, University of Calgary, Calgary, Canada; Antibe Therapeutics, Toronto, Canada
- Prof. Christine Winterbourn, University of Otago Christchurch, Centre for Free Radical Research, Christchurch, New-Zealand
Methods and equipment
Metabolomics and proteomics platform
Enzymatic activity, analysis of various metabolites and reactive sulfur species by mass spectrometry and liquid chromatography
- Thermo LTQ XL mass spectrometer
- EASY-nLC II nano HPLC
- · Thermo Scientific UltiMate 3000 HPLC with fluorescent detector
Cell and tissue culture
Investigation of human cancer cell lines – breast, lung, pancreas, melanoma
Development of xenograft models in immune deficient mice
Examination of neutrophil granulocytes isolated from human blood plasma
Processsing of mouse tissue samples for metabolomics and proteomics
- sterile cell culture hoods, incubator, hypoxic chamber, microscope, etc.
- tissue staining procedures (immunohistochemistry)
- · Braun Mikro-Dismembrator II tissue homogenizator
Gene silencing with lentiviral and CRISPR techniques
Transient and stable transfection of mammalian cell lines
Design and amplification of plasmid constructs
- rotary shaker suitable for bacterial culture
- PCR instrument
- · agarose gel electrophoresis equipment for DNA/RNA separation
SDS-PAGE and Western blot
immunoblotting of purified proteins and whole cell/tissue lysates
2D és 2D diagonal gel electrophoresis
- CBS Scientific gel electrophoresis equipment
- BioRad Mini-Protean wet Western blot equipment
- 2D gel electrophoresis equipment
- Syngene Gbox gel documentation system
- · Gel staining methods: Coomassie Brilliant Blue, Ponceau S, silver staining, fluorescent staining
Activity assays, UV-Vis spectrophometry, solution phase kinetics and mechanisms
- BioTek PowerWave XS spectrophotometer and microplate reader
- Agilent Cary 8454 UV-Vis photodiode array (PDA) spectrophotometer
- Tecan Spark 10M multimode microplate reader – fluorescence, absorbance and luminescence modules; suitable to maintain living cell cultures
Methods of in-lab or collaborative development
- ProPerDP – Protein Persulfide Detection Protocol
Experimental procedure for protein persulfide detection from purified proteins, intact cells and tissue samples
Doka et al., Science Advances (2016) 2(1):e1500968. PubMed Link
Doka et al., Methods Mol Biol..
- Quantitative LC-MS detection of low molecular weight and protein derived per/polysulfide species and reactive sulfur species (HPE-IAM labeling and suitable digestion)
Hamid et al., Redox Biology (2019) 21, 101096. PubMed Link
Akaike et al., Nature Communications (2017) 8(1), 1177 PubMed Link
- Quantitative determination of H2S from biological matrices by monobromobimane labeling
Ditroi et al., (2019) 136, 146-158. PubMed Link
High sensitivity LC-MS detection of transsulfuration metabolytes
- CBS (cystathionine beta synthase) enzymatic activity from blood plasma
Krijt et al., Journal of Inherited Metabolic Disease, (2011) 34(1):49-55. PubMed Link
- Fluorescence based MPO peroxidase activity assay from supernatant of primed neutrophils
Palinkas et al., British Journal of Pharmacology (2015) 172(6):1516-32. PubMed Link
Garai et al., Methods in Molecular Biology Beltowski J, editor Springer, in press; 2017.
- Polysulfide generation activity of MPO by customized fluorescent assay
Garai et al., Free Radical Biology and Medicine (2017) 113, 551–563. PubMed Link
- · 6th World Congress on Hydrogen Sulfide in Biology & Medicine